CRISPR associated transposases

https://drive.google.com/file/d/1j1SeEfKYQvTokt18W0YA8LGs6tpdhvhS/view

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Alzheimers Disease

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Engineering CRISPR associated transposases for use in Eukaryotes. After reading some thingys I realized there is a lot of optimism regarding using CRISPR/Cas9 for genetic engineering.  According to Dockrill (2018), a study done by the Sanger institute shows that CRISPR/Cas9 is causing many mutations and that these mutations wouldn’t be detected in a standard DNA tests.  The optimism surrounding CRISPR/Cas9 should be tempered with the stark reality that CRISPR/Cas9 gene-editing technology causes many off-target and on-target mutations including splicing disruptions and mutations several kilobases away from target sites (Kosicki et al., 2018).  Since homologous recombination repair is directly tied to mitosis it does not address every cell.   Many times, CRISPR Cas9 double-stranded cut results in the target cell using non-homologous end-joining.   Resultant from this is the formation of indels which if in a protein-coding sequence can cause frameshift mutations.  These frameshifts
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